Isolation, evaluation and purification of antibacterial peptides
نویسندگان
چکیده
Antibacterial peptides were isolated from immune haemolymph of Rhinoceros beetle (Oryctes rhinoceros L.) larvae and evaluated for antibacterial activity against bacterial pathogens, viz., Klebsiella pneumoniae, Micrococcus luteus, Bacillus subtilis, Paenebacillus macerans and Pseudomonas sp. The peptides were found to be stable upto 37°C and antibacterial activity observed at pH 7.0. The peptide were active against all the pathogens with minimum inhibitory concentrations varying between 1.56 to 6.25 μM. Protein profiling on 16% Tricine sodium dodecyl sulphate polyacrylamide gel revealed that the molecular size of two antimicrobial peptides of 17 and 9 kDa, which was also observed as two peak fractions from reverse phase high pressure liquid chromatography (RP-HPLC) exhibiting antibacterial activity. These peptides were purified by Gel filtration chromatography and RP-HPLC upto 15 and 37 fold purifications, respectively.
منابع مشابه
Purification and Characterization of an Antibacterial, Antifungal and Non Hemolytic Peptide from Rana Ridibunda
Amphibians have a large variety of antimicrobial peptides that serve as natural innate barriers limiting microbial infection or, in some instances, act as an integral component in response to inflammation or microbial infection. A novel peptide with antibacterial effects and without hemolytic activity was purified from skin secretions of Rana ridibunda by multisteps cation-exchange FPLC, revers...
متن کاملEvaluation of the Effect of Less Negatively Charged Amino Acid Substitution in Synthetic Tetramer Peptide S3 Derived from Horseshoe Crab Ambocyte on its Antibacterial Properties
Introduction: The study of the effects of synthetic peptides with antibacterial properties can provide more effective antibiotics. This study designed, expressed, and investigated the Sushi 3 tetramer peptide. Subsequently, it was compared in terms of changing antibacterial properties with another Sushi3 tetramer peptide the aspartic acid and proline amino acids of which were replaced with glyc...
متن کاملIdentification and Purification of two Mammalian Nourotoxins from Iranian Scorpion (Buthotus schach) Venom
Scorpion venoms contain of variety of peptides toxic to mammals ، insects and crustaceans. Toxic peptides are the main factors in scorpion venom causing toxicity, (their amount being 1-3%of total venom.). Most of the scorpion toxins have been isolated from the venoms of scorpions in the family Buthidae. The scorpion Buthotus Schach belonging to the Buthidae family is widely found in the weste...
متن کاملIsolation and purification of HLA-DR antigen from Daudi cell line by immunoaffinity chromatography
Introduction: The major histocompatibility complex (MHC) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals. The physiologic function of MHC molecules is the presentation of peptides to T cells. In this study, we evaluated the purification of a class II MHC molecule (HLA-DR) from a human Burkitt′s lymphoma cell line; Daudi...
متن کاملIsolation, Characterization and Antimicrobial Activity of Butea monosperma (Lam.)
The root and flowers of Butea monosperma (Lam.) were extracted with methanol. Extensive chromatographic separation and purification with the organic solvents was done. Four phytochemicals were separated and their structures were established based on various spectroscopic techniques. Isolated crude extract was subjected for antibacterial activity against gram-negative and gram-positive bacteri...
متن کامل